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perm solution  (fluidigm)


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    Structured Review

    fluidigm perm solution
    Perm Solution, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 300 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perm+solution/Maxpar+Fix+and+Perm+Buffer/bio_rxiv__64898__2026__03__21__713366-288-24-26
    Average 95 stars, based on 300 article reviews
    perm solution - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Systemic inhibition of PTPN22 augments anticancer immunity
    Article Snippet: .. On the day of data acquisition, samples were stained with 1:1000 103Rh in Maxpar Fix/ Perm solution (Fluidigm) for 30 minutes at room temperature for cell identification. .. After washing the samples in CSB, samples were washed with filtered double-distilled water and resuspended in normalization beads (EQ Beads, Fluidigm).

    Article Title: High-throughput quantitation of inorganic nanoparticle biodistribution at the single-cell level using mass cytometry.
    Article Snippet: .. Cell pellets were resuspended in staining buffer followed by antibody staining and fixing: cells were incubated with a selected antibody cocktail (anti-mouse CD45 (30-F11)-147Sm; anti-mouse CD3e (145-2C11)-152Sm; anti-mouse CD8a (53-6.7)-168Er; anti-mouse CD4 (RM4-5)-172Yb; anti-mouse CD45R/B220(RA36B2)-176Yb; anti-mouse CD11b (M1/70)-148Nd; anti-mouse Ly-6G (Gr-1) (RB6-8C5)-174Yb; anti-mouse CD11c (N418)-142Nd; anti-mouse F4/80 (BM8)-159Tb; anti-mouse NK1.1 (PK136)-170Er; anti-mouse CD64 (X54-5/7.1)-151Eu; anti-mouse CD326 (EpCAM) (G8.8)-165Ho) at 25 C for 30min, excess antibodies were removed by centrifugation, and cells were stained with cell-ID Intercalator-Ir in fix and perm solution (detailed protocol available from Fluidigm website. https://www.fluidigm.com/productsupport/cytof-helios). .. Briefly, fresh enzyme mix was prepared by dissolving 0.8mgml 1 of collagenase/dispase (Roche Diagnostics) and 0.1mgml 1 DNase I (Roche Diagnostics) in RPMI-1640 medium.

    Article Title: Circulating pre-osteoclasts are primed for osteoclast fate and synovial tissue homing in psoriatic arthritis
    Article Snippet: Cells were washed with 2ml Maxpar cell staining buffer and fixed in 1.6% formaldehyde, diluted from 16% methanol free formaldehyde (ThermoScientific, 28906) in Maxpar PBS (Standard Biotools, 201058), for 10 minutes at room temperature. .. Cells were pelleted, supernatant removed, and stained overnight at 4°C with 1ml of 125nM Cell-ID Intercalator Iridium (Standard Biotools, 201192A), in Maxpar Fix and Perm Solution (Standard Biotools, 201067). .. Cells were then transferred to 1.5ml LoBind Eppendorf’s, with care being taken to collect residual cells.

    Incubation:

    Article Title: High-throughput quantitation of inorganic nanoparticle biodistribution at the single-cell level using mass cytometry.
    Article Snippet: .. Cell pellets were resuspended in staining buffer followed by antibody staining and fixing: cells were incubated with a selected antibody cocktail (anti-mouse CD45 (30-F11)-147Sm; anti-mouse CD3e (145-2C11)-152Sm; anti-mouse CD8a (53-6.7)-168Er; anti-mouse CD4 (RM4-5)-172Yb; anti-mouse CD45R/B220(RA36B2)-176Yb; anti-mouse CD11b (M1/70)-148Nd; anti-mouse Ly-6G (Gr-1) (RB6-8C5)-174Yb; anti-mouse CD11c (N418)-142Nd; anti-mouse F4/80 (BM8)-159Tb; anti-mouse NK1.1 (PK136)-170Er; anti-mouse CD64 (X54-5/7.1)-151Eu; anti-mouse CD326 (EpCAM) (G8.8)-165Ho) at 25 C for 30min, excess antibodies were removed by centrifugation, and cells were stained with cell-ID Intercalator-Ir in fix and perm solution (detailed protocol available from Fluidigm website. https://www.fluidigm.com/productsupport/cytof-helios). .. Briefly, fresh enzyme mix was prepared by dissolving 0.8mgml 1 of collagenase/dispase (Roche Diagnostics) and 0.1mgml 1 DNase I (Roche Diagnostics) in RPMI-1640 medium.

    Centrifugation:

    Article Title: High-throughput quantitation of inorganic nanoparticle biodistribution at the single-cell level using mass cytometry.
    Article Snippet: .. Cell pellets were resuspended in staining buffer followed by antibody staining and fixing: cells were incubated with a selected antibody cocktail (anti-mouse CD45 (30-F11)-147Sm; anti-mouse CD3e (145-2C11)-152Sm; anti-mouse CD8a (53-6.7)-168Er; anti-mouse CD4 (RM4-5)-172Yb; anti-mouse CD45R/B220(RA36B2)-176Yb; anti-mouse CD11b (M1/70)-148Nd; anti-mouse Ly-6G (Gr-1) (RB6-8C5)-174Yb; anti-mouse CD11c (N418)-142Nd; anti-mouse F4/80 (BM8)-159Tb; anti-mouse NK1.1 (PK136)-170Er; anti-mouse CD64 (X54-5/7.1)-151Eu; anti-mouse CD326 (EpCAM) (G8.8)-165Ho) at 25 C for 30min, excess antibodies were removed by centrifugation, and cells were stained with cell-ID Intercalator-Ir in fix and perm solution (detailed protocol available from Fluidigm website. https://www.fluidigm.com/productsupport/cytof-helios). .. Briefly, fresh enzyme mix was prepared by dissolving 0.8mgml 1 of collagenase/dispase (Roche Diagnostics) and 0.1mgml 1 DNase I (Roche Diagnostics) in RPMI-1640 medium.



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